c-Ha-ras Containing 8-Hydroxyguanine at Codon 12 Induces Point Mutations at the Modified and Adjacent Positions1
نویسندگان
چکیده
To determine the type of mutation induced by 8-hydroxyguanine in a mammalian system, we examined the mutations induced by a synthetic i -I la-ruv protooncogene containing 8-hydroxyguanine in the second po sition of codon 12 (GGC) in NIH3T3 cells. Transaction of this gene significantly increased the number of transformed foci. The c-Ha-rC) as well as mutations of the adjacent G on the 5'-side of 8-hydroxyguanine ((.— A and G-»T).
منابع مشابه
c-Ha-ras containing 8-hydroxyguanine at codon 12 induces point mutations at the modified and adjacent positions.
To determine the type of mutation induced by 8-hydroxyguanine in a mammalian system, we examined the mutations induced by a synthetic c-Ha-ras protooncogene containing 8-hydroxyguanine in the second position of codon 12 (GGC) in NIH3T3 cells. Transfection of this gene significantly increased the number of transformed foci. The c-Ha-ras gene present in these foci was analyzed by the polymerase c...
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Synthetic c-Ha-ras genes with an analogue of an abasic site in the first or the second position of codon 12, or in the second position of codon 61 were constructed and transfected into NIH3T3 cells. The genes with the lesions in codon 12 exhibited more focus formation than a normal c-Ha-ras gene, while the gene with the lesion in codon 61 did not. Transformed cells were isolated from the foci, ...
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